"Start simulating chromosome results... Eliminate 520000 results."
"Start histone binding simulation... Eliminate 490000 results."
"Start simulating the construction of nuclei..." after layers of screening and elimination, Du Xingyu obtained more than 100 gene maps that can be constructed into dinosaur nuclei.
These maps are still flawed, and he needs to eliminate and correct them by simulation.
After another week''s work, Du Xingyu finally got a complete gene map of Tyrannosaurus Rex.
Relying on this gene map, he can synthesize genomic DNA, combine it with histones and put it into the nucleus to form a nucleus containing the genetic genes of Tyrannosaurus Rex.
This only completed 10% of the cloning of Tyrannosaurus Rex. Next, he needs to transfer the nucleus into cells to build "dinosaur cells" and induce them into pluripotent stem cells.
Some of the biotechnologies involved in this have exceeded the current scope of human technology. Du Xingyu needs to rebuild research projects and overcome them one by one.
……
In a flash, a semester has passed. By mid January, during the school''s winter vacation.
"Du Xingyu, I''ll withdraw first and see you next year." Bao Xian has packed up his salute and is ready to go home for the new year.
"Bye." Du Xingyu said goodbye to him.
In the laboratory, those team members also went home for the festival.
"Don''t you go home, Du Xingyu?" Su Yutang, Du Xingyu''s mentor, said curiously.
"I want to continue to study this subject. There are few people in the holiday laboratory, so I can use it more," Du Xingyu explained.
As a graduate student, he doesn''t really have much time to use laboratory equipment. Many equipment are shared by several people. Du Xingyu wants to take advantage of the Chinese new year to cultivate T-Rex stem cells, so as to further cultivate T-Rex embryos.
Besides, Du Xingyu has no family in this world.
"Academic research is important, so is life." Su Yutang took his granddaughter Su Ziyun in one hand and put the key in his hand in the other hand. "Your paper on gene sequencing is very good. You can try to contribute to cell!"
"Well, I will when I have time." Du Xingyu said calmly.
Su Yutang looked at him in surprise. Cell is one of the most influential journals in life science, juxtaposed with nature and science.
No matter who I tell, his paper is qualified to contribute to cell. I''m afraid I''ll be excited and can''t sleep?
Is this student a little too calm? It''s like telling an ordinary person that he won 100 million in the lottery, but he didn''t respond at all!
"Do a good job!" Su Yutang said.
"Goodbye, brother Xingyu." Su Ziyun said goodbye politely. She waved and said, "you can certainly study the dinosaur hamburger!"
Du Xingyu: "
Du Xingyu finally had a lot of time to use the high-end equipment in the laboratory to personally build the nucleus of Tyrannosaurus Rex, which is much more difficult than the scientific and technological simulator.
"Hello everyone, this is the creator vlog. Today is the 9th video." Du Xingyu still keeps updating his video log, although the number of fans is barely 100.
"In the last stage, I have constructed the nucleus of Tyrannosaurus Rex. Today, I want to transplant it into the recipient cells to cultivate real dinosaur cells."
"The operation process will not be shown... After several failures, I finally got a mature dinosaur cell."
"This cell will continue to grow, divide, and then induce it to become a stem cell."
The day after Du Xingyu sent the video, there were only a few comments.
0.05:1: "@ creator vlog, let''s change the theme. No one will watch this video."
Fihjj: "haven''t seen it for a long time. Is this man still cloning dinosaurs?"
Tomato juice: "it''s almost half a year. Why haven''t you cloned it?"
Jiang ye: "Hey, I actually met the hard core author. I''m also a student in the biology department. I feel that the author is very powerful. I''ll go and see the video in front of you!"
……
New year''s Eve, new year''s Eve, thousands of lights.
The whole country is shrouded in the joy of family harmony, and Du Xingyu is still studying in the laboratory.
He has cultivated dozens of generations of Tyrannosaurus Rex pluripotent stem cells, constantly induced, screened and determined, hoping to get a stem cell that can be used to cultivate embryos.
Thanks to the technology simulator, he saved a lot of trial and error costs. If you really let Du Xingyu try it slowly alone, I''m afraid it will be difficult to complete it all his life!
The science and technology simulator can eliminate countless possible errors in advance and generate simulation results in an instant during the test process that would have taken several years or even hundreds of years!
Under the biological microscope, Du Xingyu finally found a dinosaur stem cell that met the requirements in the cell culture dish in vitro.
"Great!" Du Xingyu was overjoyed. "We can finally cultivate dinosaur embryos!"
Inducing stem cells to develop into embryos in vitro is also a super difficult task. But Du Xingyu is not worried at all. In the science and technology simulator, he has even bred an individual Tyrannosaurus Rex.
It''s just because of the limitations of the actual experimental process, so it hasn''t been completed yet.
"If everything goes well, I can probably finish it in three months," Du Xingyu thought.
He picked up his mobile phone, and the New Year greetings from his friends were displayed on the information bar. Du Xingyu edited the message and sent it: "Happy New Year!"
……
After the winter vacation, the students returned to school.
Bao Xian rushed to the laboratory at the first time and shouted, "Du Xingyu, Du Xingyu! Where are the dinosaur embryos you cultivated?"
"Don''t tease me. I still have a week off. Listen to you, I''ll come quickly!"
In a recent chat, he learned that Du Xingyu was already cultivating T. rex embryos in vitro.
"In the embryo breeding room," Du Xingyu said with a smile.
"Let me see!" Bao Xian ignored others and immediately went to watch.
In the embryo breeding room, dozens of small embryos are taking shape. Du Xingyu used biological culture medium to simulate the situation of the mother for cultivation.
Because dinosaurs have long been extinct, it is difficult to find a suitable surrogate host. Du Xingyu originally wanted to use birds as the surrogate host of dinosaur embryos, but he found that the egg size of Tyrannosaurus rex was too large, and only ostriches could barely give birth to it in the world.
But what vertebrates express during development will be affected by maternal genes and proteins. Du Xingyu has simulated it countless times with a scientific and technological simulator, and most of them failed.
Only a few times, the simulated surrogate ostrich gave birth to a half bird and half dinosaur monster. This little monster also broke its shell and died in a few minutes.
Therefore, after comprehensive consideration, Du Xingyu chose to completely simulate the situation of dinosaur mother to provide a development environment for Tyrannosaurus Rex embryos.
"Is this true?" Bao Xian could hardly believe that these embryos were Tyrannosaurus Rex embryos. These embryos had just begun to develop. It would take decades to develop into a Tyrannosaurus Rex egg.
"Of course it''s true," said Du Xingyu, "but I expect that only three or five of the dozens of samples can eventually develop into eggs. Only one or two can hatch smoothly."
Du Xingyu is conservative. According to the simulation results on the science and technology simulator, the survival rate of these embryos should be higher.
"Shit, don''t tease me. If Tyrannosaurus Rex embryo, you''ll be bigger than a cow!" Bao Xian said. "You did what scientists all over the world didn''t do!"
"I''m not sure yet," said Du Xingyu.
"No, I still don''t believe it!" Bao Xian shook his head again and again. "Even if you found the Tyrannosaurus Rex gene map, you can''t encode its nucleus and stem cell cultivation in such a short time... Can''t you make a mistake once?"
"How much work and how many worldwide problems can you complete alone?"
"Is this another animal embryo? Say, did you change the subject secretly?"
"Believe it or not." Du Xingyu couldn''t explain. He couldn''t tell him to take the black technology plug-in with him?
……
"Are these really Tyrannosaurus Rex embryos?" not only Bao Xian, but also other members of the laboratory wondered.
Pang Yan wondered, "it doesn''t make sense, this..."
"Younger martial brother, is this another animal embryo? It looks like some kind of bird." a senior sister said.
"Du Xingyu, you teased us when we first came." Yang Mu said, "I don''t believe it can really cultivate a Tyrannosaurus Rex!"
Du Xingyu said helplessly, "now the embryo has just begun to develop... It will be seen in a while."
"Your T-Rex gene map can''t prove to be true! There is no living T-Rex in the world!" someone said.
"Impossible, impossible!" said a senior doctor named Zhu LianBo. "If this is a dinosaur embryo, I can trust you!"
"Don''t believe me," said Du Xingyu. "Well, if I really cultivate dinosaur embryos, you can treat me to dinner. If I fail, I''ll treat you to dinner."
"OK!" Yang Mu said, "that''s it!"
"OK, then you have to cultivate it well!" said Pang Yan, Zhu LianBo and others.
Du Xingyu''s cultivation of dinosaur embryos not only surprised and puzzled the whole laboratory, but even Su Yutang came to see it in person.
He carefully read some experimental records of Du Xingyu and said very seriously, "Du Xingyu, whether this experiment can be completed or not, you have said it has been successful!"
"Coding their own genome, inducing stem cells to cultivate embryos, and this technology alone is enough to startle the whole biological world!"
"From now on, your project is the top priority of our laboratory. All experimental projects make way for it!"
"I hope you can get more research results!"
"Thank you, teacher!" Du Xingyu thanked.
Su Yutang''s explanation made the members of the whole laboratory even more surprised. Is Du Xingyu really so powerful? The teacher paid so much attention to Du Xingyu''s project. Is it true that the cloned Tyrannosaurus rex has a head?