Institute of cryptococcosis, 2333 Jiangzhou science and technology park.
It has been more than half a year since it was founded last year.
But so far, the Institute has achieved nothing.
The reason has also been said before that it is too difficult to clone cells, because the content of antigenic B cells in serum is too low.
After isolation of B cells, the sensitivity and specificity of sequence amplification and determination are also very high.
Even if the sequence is amplified and the antibody is expressed, the neutralization effect of the antibody needs further identification.
In short, it is extremely difficult to replicate antigenic B cells.
Of course, all of the above are for ordinary people.
Zhou Wen is not included.
He has now upgraded several subjects to lv4, and this task is almost ready to start.
This task brings Zhou Wen not only experience, but also some additional things, such as his influence in the academic circles, which other tasks can not bring him.
On the afternoon of May 5, Zhou Wen officially started cell cloning research.
The first step is to isolate B cells.
This is extremely difficult for others, but it is a piece of cake for Zhou Wen, who has the eye of true vision.
First of all, his eyes can directly see the antigenic B cells in the serum;
secondly, the upgrading of biological immunology to lv4 has brought him not only a simple amount of knowledge, but also a deeper understanding of antigenic B cells. He has mastered a set of unique separation methods.
These techniques are independent of the existing biological knowledge.
One week later, Zhou Wen successfully isolated cryptococcosis antigenic B cells.
Li Feng and Fan Bo, who have been criticizing Zhou Wen in their hearts, almost didn''t stare out in shock.
The other 13 Ph.D. researchers in the lab were also shocked.
Everyone''s respect for Zhou Wen is like a torrent of water, continuous, and like the Yellow River flooding, a hair out of control.
But no one cheered!
This is just the first step in the long march.
Next comes the play sequence amplification!
This is also very difficult for ordinary people, because antigenic B cells are too few, and the failure rate is as high as 99% when amplification!
And if you fail, you''ll probably never have another chance.
But this is not a big problem for Zhou Wen.
Don''t forget, what did Zhou Wen start with? DNA extraction and amplification!
In the world of DNA extraction and PCR, he thinks that he is second, and no one dares to claim to be the first.
Of course, Zhou Wen didn''t dare to take it lightly. He still kept up his spirits and went all out.
Because there are too few specimens.
If it fails, the isolated B cells will be invalid, and it will take another week to separate again, and it may not be successful at one time!
It''s similar to the mouse DNA amplification program, except that he redesigned the primers himself.
Several simulation experiments have been carried out.
Six days later, Zhou Wen successfully amplified a sufficient number of antigenic B cells, and after product analysis, confirmed that the amplification was successful!
There is no shortage of research samples.
The whole lab is boiling!
Everyone looked at Zhou Wen like a God.
If one-time isolation of antigenic B cells belongs to the category of human beings, then it is completely non-human technology to amplify antigenic B cells at one time without operation experience and without failure.
At this moment, Zhou Wencheng for their spiritual faith!
Even if he said he was an alien now, they believed it!
The next step is the determination of antibody expression.
Antibody expression is a multi-step process that starts from DNA to mRNA transcription and reaches high tide when mRNA is translated into protein
It saves 30000 words.
Not surprisingly, the sensitivity of antibody expression is naturally a one-time pass.
Li Feng and a group of doctors were not surprised, and even took it for granted.
The next step is the most important and final step to test the neutralization effect of antibody.
Because "antibody" can only recognize target antigen, but not kill or exclude target antigen.
And "neutralization effect" is that antibody and antigen combine to produce precipitation or cell group, and then be phagocytized by phagocytes, and then kill the antigen clean!
If the neutralization test is successful, it means that the vaccine for cryptococcosis has been successfully manufactured.
……
Xiangya Hospital, infection department director''s office.
Cheng Yanqing is writing the experiment report when the telephone on the desk rings.
She picked it up and looked at it. It was pan Renfeng, a member of the virus exchange family and director of the infection department of the harmony hospital."What''s up, director Pan?"
"Zhou said that the antigenic B cells they amplified had been tested for antibody expression and would soon be tested for neutralization effect."
Cheng Yanqing Leng for a moment, and then he was shocked and said, "what do you say?"
"I said Zhou Wen and they have isolated and amplified the antigenic B cells of cryptococcosis, and we are going to test the neutralization effect."
"Impossible!" Cheng Yanqing said categorically, "I called the Research Institute at the beginning of the month, and there is still doing basic research. It''s impossible."
Pan Renfeng said: "this is not what I said, this is Zhou Wen himself said, you go to the group and have a look."
Hearing the speech, Cheng Yanqing immediately opened the wechat group. As a result, he really saw Zhou Wen''s speech.
It was Xia''s old house who asked Zhou Wen what he was doing recently. Zhou Wen said casually that he was cloning antigenic B cells of cryptococcosis.
Then the old house of the Xia family asked him how his research was going?
Zhou said the neutralization effect test would start immediately.
Zhou Wen''s words, the group of those diving screen all the big bang out.
No one can believe it.
But Zhou Wen in drying out a few test data table, the group suddenly fried.
Cheng Yanqing points to open the test data to see, shocked to the extreme!
She wondered if it was April Fool''s day?
How could such a thing exist?
She called Li Feng at the beginning of the month. He groaned and said that there was no progress. The words revealed complaints about Zhou Wen.
But now
Cheng Yanqing immediately called Zhou Wen, who was on the phone.
Cheng Yanqing did not hang up the phone, but had been waiting.
About a few dozen seconds later, the phone was connected.
"Zhou Wen, what''s going on?"
"That I can''t explain clearly for a moment and a half. Well, director Cheng, if you have time, come to Jiangzhou tomorrow and have an interview. "
Cheng Yanqing had too many questions to ask, but he didn''t know where to start. He had to hang up for a while.
But how could Cheng Yanqing wait until tomorrow?
Without saying a word, he left for Jiangzhou immediately.
On the way, I called Li Feng, the person in charge of the laboratory.
Finally, we got through.
Almost, Li Qingfeng didn''t come back to talk about it for a long time.
"Dudu Dudu -"
finally, Cheng Yanqing was awakened by the sound of the horn behind him. At this time, he found that he had stopped at the intersection of traffic lights.
Cheng Yanqing took a deep breath and asked, "why don''t you tell me about such a big thing?"
Li Feng said: "because Zhou said that the process is not important, the important thing is the result. If the neutralization effect test is not passed, all the previous sweat and efforts are meaningless, so there is no need to let everyone know."
Cheng Yanqing:
Is this human language?
Successful isolation of antigenic B cells is a great feat. It is also a very important reference for the isolation of antigenic B cells from other rare diseases!
In her opinion, this technology alone is worth three lancets!
How can you say it''s meaningless?
As for the latter sequence amplification, it is also very important.
"This little fellow, what a shame
Cheng Yanqing said something, but he couldn''t help laughing.
……
Sunday, May 26, 2019.
At 9:30 a.m., Jiangzhou Institute of cryptococcosis conducted an open test on the neutralization effect of antigenic B cells of cryptococcosis.
Some good friends and leaders familiar with Zhou Wen visited the site.
Such as pan Renfeng, Cheng Yanqing, Gu Yunwen, Mu Yuanqing, Lin huaidong, Zhang Wen, as well as several leaders of Jiangzhou Medical College and respected experts and professors.
The results were not unexpected.
In the eyes of the public, the antibody of cryptococcosis and the antigen of cryptococcosis combine to produce cell groups, which are swallowed up by phagocytes!
"Pa Pa Pa Pa --"
in the observation room outside the laboratory, there was a warm applause!
"Congratulations to director Cheng. You are really amazing."
"It''s a great invention! Director Cheng, how are you! "
“……”
In the face of praise from a group of old friends, Cheng Yanqing jokingly said, "OK, all right, you''ll be less flattering here! I am also a leader. All the research work is done by Zhou Wen, which has nothing to do with me. "
Experts at the scene all know what''s going on, so everyone is laughing.
It doesn''t matter if friends make fun of each other, but they are not so cheeky when they really try to grab other people''s academic achievements!And this can''t be taken.
Some of the core technical processes inside, when the experimental recovery can not be done, the shame will be lost to grandma''s house.
The experts were sorry, but one of the leaders of Jiangzhou Medical College laughed and said, "director Cheng is too modest!
Xiao Zhou''s outstanding ability is undeniable, but we can''t ignore the role of leadership. Without the leadership''s guidelines and good scheduling, their young people can only turn around like flies without heads, right? "
Seeing the leader''s serious face, Cheng Yanqing was really embarrassed. He said to himself, "I''m not modest. Please don''t take me with me if you brag!"
Seeing the expression on Cheng Yanqing''s face, pan Renfeng, Lin huaidong, Zhang Wen and others all burst into laughter.
But the big bellied leader of Jiangzhou Medical College, in his heart, was baffled and said to himself, "is my words very funny?"
However, he did not dare to question or even to show his face.
Pan Renfeng, Gu Yunwen, Lin huaidong, Zhang Wen, etc., who is not a big man in the domestic medical system?
The chief can talk directly to the leader.
At his level, it is not enough to see
www.novelhold.com , the fastest update of the webnovel!